ABSTRACT
Dengue fever has become one of the deadliest infectious diseases and requires the development of effective antiviral therapies. It is caused by members of the Flaviviridae family, which also cause various infections in humans, including dengue fever, tick-borne encephalitis, West Nile fever, and yellow fever. In addition, since 2019, dengue-endemic regions have been grappling with the public health and socio-economic impact of the ongoing coronavirus disease 19. Co-infections of coronavirus and dengue fever cause serious health complications for people who also have difficulty managing them. To identify the potentials of mangiferin, a molecular docking with various dengue virus proteins was performed. In addition, to understand the gene interactions between human and dengue genes, Cytoscape was used in this research. The Kyoto Encyclopedia of Genes and Genomes software was used to find the paths of Flaviviridae. The Kyoto Encyclopedia of Genes and Genomes and the Reactome Pathway Library were used to understand the biochemical processes involved. The present results show that mangiferin shows efficient docking scores and that it has good binding affinities with all docked proteins. The exact biological functions of type I interferon, such as interferon-α and interferon-ß, were also shown in detail through the enrichment analysis of the signaling pathway. According to the docking results, it was concluded that mangiferin could be an effective drug against the complications of dengue virus 1, dengue virus 3, and non-structural protein 5. In addition, computational biological studies lead to the discovery of a new antiviral bioactive molecule and also to a deeper understanding of viral replication in the human body. Ultimately, the current research will be an important resource for those looking to use mangiferin as an anti-dengue drug. Supplementary Information: The online version contains supplementary material available at 10.1007/s43450-022-00258-6.
ABSTRACT
The world has witnessed and still suffers from the novel coronavirus. Scientists are working hard to find medicine that can cure the coronavirus, but unfortunately, they are not having anything concrete with them. Thus, the vaccine is only a panacea to prevent hazardous effects by covid-19. Secondly, social distancing also plays a vital role to combat coronavirus till an effective solution would be not found. Despite running the third wave of the corona, most of the people have to visit crowded-place such as shopping malls, restaurants, etcetera, where visitors have to register their personal details. Until now, the method, which is based on pen and paper, used to take too much time in recording every visitor's information at such places. So, this research paper is focusing on to digitalize the process of recording the information visitors. A flutter application namely 'Visitors' is developed to make this whole process smooth and time efficient. This Application provides a way of keeping records of visitors at such places where usually incessant people are visiting every day. Therefore, this app provides contact less and technological solutions for inserting, keeping and maintaining the data of strangers. By using this application, visitors can generate a QR(Quick Response) code of their personal and vaccination details, therefore, they don't need to wait in a queue outside of such places to register themselves by traditional method. Thus, they do not come in contact with corona patients as it provides contact less and astute methods. © 2022 IEEE.
ABSTRACT
Dengue fever has become one of the deadliest infectious diseases and requires the development of effective antiviral therapies. It is caused by members of the Flaviviridae family, which also cause various infections in humans, including dengue fever, tick-borne encephalitis, West Nile fever, and yellow fever. In addition, since 2019, dengue-endemic regions have been grappling with the public health and socio-economic impact of the ongoing coronavirus disease 19. Co-infections of coronavirus and dengue fever cause serious health complications for people who also have difficulty managing them. To identify the potentials of mangiferin, a molecular docking with various dengue virus proteins was performed. In addition, to understand the gene interactions between human and dengue genes, Cytoscape was used in this research. The Kyoto Encyclopedia of Genes and Genomes software was used to find the paths of Flaviviridae. The Kyoto Encyclopedia of Genes and Genomes and the Reactome Pathway Library were used to understand the biochemical processes involved. The present results show that mangiferin shows efficient docking scores and that it has good binding affinities with all docked proteins. The exact biological functions of type I interferon, such as interferon-α and interferon-β, were also shown in detail through the enrichment analysis of the signaling pathway. According to the docking results, it was concluded that mangiferin could be an effective drug against the complications of dengue virus 1, dengue virus 3, and non-structural protein 5. In addition, computational biological studies lead to the discovery of a new antiviral bioactive molecule and also to a deeper understanding of viral replication in the human body. Ultimately, the current research will be an important resource for those looking to use mangiferin as an anti-dengue drug. Graphical Supplementary Information The online version contains supplementary material available at 10.1007/s43450-022-00258-6.
ABSTRACT
Dengue fever is a tropical endemic disease that is transmitted by the female Aedes aegypti mosquitoes around the world. In dengue patients, the severe vascular leak, bleeding and organ failure underlines the severity of this disease. It affects 100 million people each year and half a million cases of dengue hemorrhagic fever are recorded worldwide. In addition, the combined infections of COVID-19 and dengue fever cause serious health problems for those infected. Because polyphenolic compounds have the ability to regulate and restore the immune system, the intervention of biological tools is required to find an accurate polyphenolic compound from plants to combat these viral diseases. In this computational study, we used a range of software to explore the anti-dengue potential of the selected polyphenols, while the database was also used to explore diseased enzymes and drug targets in the dengue pathways of Homo sapiens. All examined polyphenols showed excellent docking values after molecular docking and also showed remarkable hydrogen bond interactions between the ligand and the dengue protein. Among the polyphenols tested, (R)-(+)-rosmarinic acid has a docking score of −8451 with an energy value of −59,860. After that, curcumin has the second best docking score of −8.221, followed by D - (+) - catechin (−7.042) and epicatechin (−7.069). In addition, molecular dynamic simulations demonstrate that the complex of (R) - (+) - rosmarinic acid and dengue protein has a solid understanding of the precise interactions. The current study suggests that this is the first report of such compounds against the dengue virus, despite extensive research on these substances in previous research. Moreover, we expect these polyphenols to show promising effects when used in antiviral research such as in-vitro and in-vivo studies. In addition, toxicological studies such as adsorption, distribution, metabolism, and excretion are required before further exploration of the polyphenols, as this will help to understand the biological processes of these potential polyphenols. © 2022 Elsevier Inc.
ABSTRACT
Introduction: Hand hygiene being a simple action, compliance among health care workers is < 40%. one such effort is introduction of "my moments for hand hygiene." Aim & Objective: To assess the Knowledge, Attitude and Practise of hand hygiene practices among interns. Material & Methods: Study was conducted during Covid19 period (April to June 2020.) A questionnaire based on WHO's concept of "Five Moments for Hand Hygiene" was used to evaluate awareness of indications for hand hygiene and compliance was observed during Objective Structured Clinical Examination (OSCE) sessions. Observations & Results: 105 interns were taken for the study. 66 males (63%) and 39 females (37%) participated voluntarily. 5 internees three males and two females have not cooperated during the study. Discussion: 57.3% of positive indications for hand hygiene were correctly identified & 42.53% students were not sure about these moments which had similar findings with Mann and Wood.